Porcine viral diarrhea, PTGV, PEDV, PoRV Triplex Real-time PCR Kit

This PTGV, PEDV, PoRV Triplex Real-time PCR Kit uses Taqman real-time fluorescent probe technology to detect conserved genes of porcine infectious gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus, and can be applied to the detection of pig-derived samples such as pig feces, swabs, blood, saliva, lymph nodes, spleen, kidneys, and non-porcine source samples such as feed and environment.

Porcine viral diarrhea can cause great damage to the pig industry. However, multiple infections have contributed to the poor control of diarrhea, which has also resulted in great difficulties in determining the main pathogenic factors. The viruses that cause porcine diarrhea disease are diverse, including porcine epidemic diarrhea virus (PEDV) [3–5], porcine deltacorona virus (PDCoV), porcine transmissible gastroenteritis virus (TGEV) , porcine teschvirus (PTV), bovine viral diarrhea virus (BVDV), porcine rotavirus (PoRV), porcine sapelovirus (PSV), porcine kobuvirus (PKoV), porcine astrovirus (PAstV), porcine torovirus (PToV), porcine sapovirus (PoSaV), etc. An infection with any of these viruses can develop into similar clinical symptoms, including severe diarrhea and dehydration, and in some cases, multiplex infections of these pathogens are common. However, it remains difficult to distinguish these pathogens in clinic.

Key facts of this PTGV, PEDV, PoRV Triplex Real-time PCR Kit

• Ready to use kits for veterinary labs
• No special instrument required
• Only less than 60min to get the result
• Low cost with high sensitivity
• Used to distinguish the 3 major viruses in porcine viral diarrhea

Performance of the PTGV, PEDV, PoRV Triplex Real-time PCR Kit

This PTGV, PEDV, PoRV Triplex Real-time PCR Kit uses Taqman real-time fluorescent probe technology to detect conserved genes of porcine infectious gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus, and can be applied to the detection of pig-derived samples such as pig feces, swabs, blood, saliva, lymph nodes, spleen, kidneys, and non-porcine source samples such as feed and environment.

PTGV, PEDV, PoRV Triplex Real-time PCR Kit Components

• Negative Control
• Positive Control
• Enzyme Mix
• Sample buffer

Extended Reading

• The Pig Site, Rotavirus page
• MSD Veterinary Manuals, Rotavirus 
• Shi, Y., Li, B., Tao, J., Cheng, J., & Liu, H. (2021). The Complex Co-infections of Multiple Porcine Diarrhea Viruses in Local Area Based on the Luminex xTAG Multiplex Detection Method. Frontiers in veterinary science, 8, 602866. https://doi.org/10.3389/fvets.2021.602866