Mycoplasma hyopneumoniae Real-time PCR Kit

Mycoplasma hyopneumoniae Real-time PCR Kit uses Taqman real-time fluorescent probe technology to detect mycoplasma porcine pneumoniae conservative gene, which can be applied to the detection of mycoplasma purine nucleic acid in pig nasal mucus, lungs, saliva and non-pig source samples such as feed and environment.

Mycoplasmal pneumonia of swine (MPS, also referred to as “enzootic pneumonia,” EP) is a common, widely distributed disease that occurs throughout the year. It is restricted to swine and occurs in all major swine-raising countries. MPS often is more apparent in chronic form on premises where there is continuous flow production and management, and where husbandry and environmental conditions are poor. MPS may cause fairly acute and severe disease in immunologically naïve pigs in age-segregated systems. It may affect pigs early after weaning, when passive immunity has waned, but more commonly occurs in grower and finisher (80-200 lbs) stages. Mycoplasmal pneumonia often interacts with and contributes to other respiratory diseases and is considered to have a central role in porcine respiratory disease complex (PRDC). Secondary bacterial infections are common.

Mycoplasma hyopneumoniae is a common cause of pneumonia in pigs worldwide. It also frequently leads to subclinical infection that causes lung lesions that can be detected post-mortem. Clinical signs, if present, are often a dry cough and reduced growth. Diagnosis may be based on clinical signs, characteristic lesions, and confirmation by PCR assay. Partial disease control can be achieved via improved management practices, antimicrobial treatment, and vaccination.

Key facts of this Mycoplasma hyopneumoniae Real-time PCR Kit

• Ready to use kits for veterinary labs
• No special instrument required
• Only less than 60min to get the result
• Low cost with high sensitivity

Performance of the Mycoplasma hyopneumoniae Real-time PCR Kit

This kit uses Taqman real-time fluorescent probe technology to detect mycoplasma porcine pneumoniae conservative gene, which can be applied to the detection of mycoplasma purine nucleic acid in pig nasal mucus, lungs, saliva and non-pig source samples such as feed and environment.

Mycoplasma hyopneumoniae Real-time PCR Kit Components

• Negative Control
• Positive Control
• Enzyme Mix
• Sample buffer

Extended Reading

• Iowa State University, Swine Manual, Mycoplasma hyopneumoniae
• MSD Veterinary manual, Mycoplasma hyopneumoniae
• Streck, A. F., & Truyen, U. (2020). Porcine Parvovirus. Current issues in molecular biology, 37, 33–46. https://doi.org/10.21775/cimb.037.033